The Chemosensitizing Effect of Portulaca oleracea Methanol Extract on Human Colorectal and Lung Cancer Cells Against Irinotecan

Cancer is a major health problem and it has been causing great damage in recent years worldwide. Serious side effects of chemotherapeutics used cancer treatment make natural products valuable. Combined use chemotherapy drugs herbal an important approach reducing revealing more effective treatment. In this study, the methanolic extract from Portulaca oleracea (POM) alone combination with Irinotecan were investigated against human colorectal carcinoma cell line HCT-116 lung adenocarcinoma A549. The antiproliferative/anti-colony formation determined using WST-1 assay clonogenic assay. Cell death detection Elisa was employed to exhibite triggered apoptosis, quantitative real time polimerase chain reaction (qRT-PCR) performed evaluate mRNA expression mTOR, Akt PTEN genes, which are significant modulators apoptotic survival pathways. It that 72 h increasing concentrations POM (0-1000 mg mL-1) decreased viability HCT116 A549 cells dose-dependent manner. Also, exhibited synergistic both lines. suppressed colony forming capacity by 84% 79%, respectively. also altered gene expressions cells, Irinotecan. This study shows phytotherapeutic effect P. needs be studied intensively.